Nexera X4 Ultra High-Performance Liquid Chromatograph - Features
Ultra High-Performance Liquid Chromatograph
Ultra-Sharp Peaks
Higher sensitivity and outstanding resolution through minimized peak dispersion
The Nexera X4 is engineered to deliver ultra-sharp peaks by minimizing system-derived peak broadening throughout the flow path. Its proprietary Nexflow technology reduces extra-column dispersion without changing the internal diameter or length of the tubing, helping laboratories fully realize UHPLC column performance while maintaining practical robustness and reducing the risk of blockages. This low-dispersion design supports outstanding chromatographic separation, particularly in applications where peak broadening outside the column can limit resolution, and improves theoretical plate numbers for weakly retained compounds in isocratic analysis.
Key Performance Benefits
- Maximized Column Performance: Nexflow technology reduces extra-column dispersion, enabling UHPLC columns to deliver their full separation potential and helping preserve peak integrity from column to detector.
- Higher Sensitivity and Stable Detection: A newly designed PDA detector with a capillary cell reduces peak broadening, minimizes solvent-derived refractive index effects and contributes to stable baselines with high sensitivity across a wide concentration range.
- Low-Dispersion Injection for Demanding Samples: The SIL-40C X4 autosampler combines a low-internal-volume high-pressure valve, low-dispersion injection mode and effective rinsing, making it suitable for both highly sensitive analyses and challenging high-concentration samples.
Ultra-Fast Analysis
Higher throughput and reliable results through consistent, high-speed gradient performance
Ultra-fast analysis on the Nexera X4 is driven by advanced fluid control technologies designed to maintain precision even under demanding high-pressure conditions. A binary gradient pump with four independently actuated plungers and a pressure feedback mechanism improves solvent delivery responsiveness, reduces pulsation and supports stable baselines during fast gradients. This enables the system to deliver highly consistent ultra-fast gradients, helping laboratories increase throughput while maintaining reliable retention behaviour and analytical consistency.
Method flexibility is further expanded through optional solvent switching and online degassing, which support rapid mobile phase selection and more efficient method screening. Together, these capabilities help accelerate both method development and routine analysis without compromising confidence in the data. By combining dramatically shorter analysis times with excellent retention time reproducibility, the Nexera X4 delivers higher productivity and reliable reproducibility through consistent ultra-fast gradients.
Ultra-Low Solvent Consumption
Lower solvent use and excellent separation through analytical miniaturization
Miniaturized LC workflows place especially high demands on instrument performance. To fully realize the benefits of narrow-bore columns, a system must deliver precise micro-injection, accurate solvent delivery at very low flow rates and tight control of peak broadening throughout the flow path. The Nexera X4 is designed to meet these requirements, giving laboratories the performance needed for analytical miniaturization without compromising separation quality.
Performance for Analytical Miniaturization
- Reliable Micro-Injection: A high-precision measuring pump enables excellent repeatability even at injection volumes of 1 µL or less, supporting accurate and dependable analytical miniaturization.
- Precise Low-Flow Solvent Delivery: The Nexera X4 provides accurate solvent delivery even at micro flow, while its small internal volume supports responsive gradient analysis with narrow-bore columns.
- Consistent Separation Quality: Proprietary Nexflow technology reduces peak broadening, helping maintain chromatographic integrity even under miniaturized conditions. Used with Shimadzu’s 1.0 mm I.D. narrow-bore columns, the system helps significantly reduce solvent consumption while maintaining excellent analytical performance.
Best Partner for Mass Spectrometry
Maximized LCMS performance for high-speed, high-sensitivity analysis
"For laboratories combining liquid chromatography with mass spectrometry, the Nexera X4 serves as a high-performance LC front end designed to preserve chromatographic quality from separation through detection. Dedicated Nexflow Tubing for MS reduces peak broadening between the column outlet and the MS interface, helping the system transfer the ultra-fast, high-separation capabilities of the Nexera X4 more effectively to the mass spectrometer. This enables LC-MS workflows that combine speed, sensitivity and reproducibility in a single platform.
In combination with Shimadzu LCMS systems, the Nexera X4 supports fast, high-sensitivity analysis with stable and reproducible results. Precise gradient control and low-dispersion flow paths help maximize throughput and analytical confidence in demanding applications and high-throughput workflows. Together, these technologies make the Nexera X4 an ideal partner for laboratories seeking combinations that maximize mass spectrometer performance."